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individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774  (R&D Systems)


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    R&D Systems individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774
    Individual Enzyme Linked Immunosorbent Assay Elisa Kits Kits R D Systems Inc Dy1774, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duoset+elisa+kit+r+d+systems/Canine+CCL2%2FMCP-1+DuoSet+ELISA/pmc13108725-70-25-31
    Average 93 stars, based on 4 article reviews
    individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774 - by Bioz Stars, 2026-10
    93/100 stars

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    Electron Microscopy:

    Article Title: Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-streptomycin-L-glutamine (100X) Gibco Cat# 10378016 Amphotericin B Sigma-Aldrich Cat# A9528 RPMI 1640 medium Gibco Cat# 11875093 Insulin solution human Sigma-Aldrich Cat# I9278 Apo-transferrin human Sigma-Aldrich Cat# T2036 Advanced DMEM F12 Gibco Cat# 12634010 HEPES Gibco Cat# 15630106 Non-essential amino acids solution Gibco Cat# 11140050 Sodium pyruvate Gibco Cat# 11360070 Trypsin Sigma-Aldrich Cat# T4799 Sucrose Sigma-Aldrich Cat# S0389 DPBS, calcium, magnesium Gibco Cat# 14040133 Cesium chloride Sigma-Aldrich Cat# 20996 SeaKem ME agarose Lonza Cat# 50010 Medium 199 (10X) Gibco Cat# 11825015 Sodium bicarbonate Gibco Cat# 25080094 Neutral red solution Sigma-Aldrich Cat# N2889 BACTO agar BD Cat# 214010 Psoralen Sigma-Aldrich Cat# P8399 Ondansetron Selleckchem Cat# S1390 Formalin 10% Neutral Buffered Solution Research Products International Cat# F10800 Tissue-Tek O.C.T. .. Compound SAKURA Cat# 4583 Albumin, Bovine Fraction V [BSA] Research Products International Cat# A30075 Triton X-100 Sigma-Aldrich Cat# T8787 DL-dithiothreitol Sigma-Aldrich Cat# 43815 UltraPure 0.5M EDTA Invitrogen Cat# 15575020 Matrigel Corning Cat# 356234 Y-27632 dihydrochloride R&D Cat# 1254 SB 431542 R&D Cat# 1614 Gastrin Sigma-Aldrich Cat# G9145 A83-01 Sigma-Aldrich Cat# SML0788 DAPT Selleckchem Cat# S2215 Mouse EGF R&D Cat# 2028-EG Paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 15710 Collagen Sigma-Aldrich Cat# C5533 TrypLE Express Enzyme Gibco Cat# 12604013 Human EGF Invitrogen Cat# A42556 Critical commercial assays The human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA kit R&D Systems Cat# DY1598B05 Serotonin ELISA kit IBL International Cat# RE59121 RNeasy Plus Mini Kit QIAGEN Cat# 74136 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 SYBR Green qPCR ReadyMix Sigma-Aldrich Cat# KCQS00 TaqMan assays Applied Biosystems Cat# 4444557 Deposited data scRNA-seq datasets This study GSE288639 Experimental models: Cell lines African green monkey: MA104 cells ATCC Cat# CRL-2378.1 Mouse: L929 cells ATCC Cat# CCL-1 (Continued on next page) Cell Host & Microbe 33, 408–419.e1–e8, March 12, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: GOT1 cells Dr. Lennart Svensson N/A Human: HT-29 cells ATCC Cat# HTB38 Experimental models: Organisms/strains 129S1/SvImJ The Jackson Laboratory Strain #: 002448 Ifnar1-/- Ifngr1-/- Virgin laboratory39 N/A 129S6/SvEv-Stat1tm1Rds Taconic Biosciences Strain #: 2045 C57BL/6J The Jackson Laboratory Strain #: 000664 B6.Cg-Ifngr1tm1Agt Ifnar1tm1.2Ees/J The Jackson Laboratory Strain #: 029098 Ifnar1-/- Ifnlr1-/- Kotenko laboratory40 N/A B6.129S7-Ifngr1tm1Agt/J The Jackson Laboratory Strain #: 003288 Ifnlr1-/- Kotenko laboratory40 N/A Ifnlr1fl/fl Ding laboratory N/A Ifnlr1fl/fl Vil-Cre Ding laboratory N/A Mavsfl/fl Baldridge laboratory N/A Mavsfl/fl Alpi-CreERT2 Baldridge laboratory N/A Oligonucleotides Home designed qRT-PCR primers, see Table S1 This paper N/A Mouse Nlrc5 TaqMan primer Thermo Fisher Scientific Cat# Mm01243039_m1 Mouse Tnfsf10 TaqMan primer Thermo Fisher Scientific Cat# Mm01283606_m1 Software and algorithms PRISM (version 10.3.0) Graphpad https://www.graphpad.com FIJI (version 2.14.0/1.54f) ImageJ https://fiji.sc Other Transwells Corning Cat# 3413

    Enzyme-linked Immunosorbent Assay:

    Article Title: Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-streptomycin-L-glutamine (100X) Gibco Cat# 10378016 Amphotericin B Sigma-Aldrich Cat# A9528 RPMI 1640 medium Gibco Cat# 11875093 Insulin solution human Sigma-Aldrich Cat# I9278 Apo-transferrin human Sigma-Aldrich Cat# T2036 Advanced DMEM F12 Gibco Cat# 12634010 HEPES Gibco Cat# 15630106 Non-essential amino acids solution Gibco Cat# 11140050 Sodium pyruvate Gibco Cat# 11360070 Trypsin Sigma-Aldrich Cat# T4799 Sucrose Sigma-Aldrich Cat# S0389 DPBS, calcium, magnesium Gibco Cat# 14040133 Cesium chloride Sigma-Aldrich Cat# 20996 SeaKem ME agarose Lonza Cat# 50010 Medium 199 (10X) Gibco Cat# 11825015 Sodium bicarbonate Gibco Cat# 25080094 Neutral red solution Sigma-Aldrich Cat# N2889 BACTO agar BD Cat# 214010 Psoralen Sigma-Aldrich Cat# P8399 Ondansetron Selleckchem Cat# S1390 Formalin 10% Neutral Buffered Solution Research Products International Cat# F10800 Tissue-Tek O.C.T. .. Compound SAKURA Cat# 4583 Albumin, Bovine Fraction V [BSA] Research Products International Cat# A30075 Triton X-100 Sigma-Aldrich Cat# T8787 DL-dithiothreitol Sigma-Aldrich Cat# 43815 UltraPure 0.5M EDTA Invitrogen Cat# 15575020 Matrigel Corning Cat# 356234 Y-27632 dihydrochloride R&D Cat# 1254 SB 431542 R&D Cat# 1614 Gastrin Sigma-Aldrich Cat# G9145 A83-01 Sigma-Aldrich Cat# SML0788 DAPT Selleckchem Cat# S2215 Mouse EGF R&D Cat# 2028-EG Paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 15710 Collagen Sigma-Aldrich Cat# C5533 TrypLE Express Enzyme Gibco Cat# 12604013 Human EGF Invitrogen Cat# A42556 Critical commercial assays The human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA kit R&D Systems Cat# DY1598B05 Serotonin ELISA kit IBL International Cat# RE59121 RNeasy Plus Mini Kit QIAGEN Cat# 74136 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 SYBR Green qPCR ReadyMix Sigma-Aldrich Cat# KCQS00 TaqMan assays Applied Biosystems Cat# 4444557 Deposited data scRNA-seq datasets This study GSE288639 Experimental models: Cell lines African green monkey: MA104 cells ATCC Cat# CRL-2378.1 Mouse: L929 cells ATCC Cat# CCL-1 (Continued on next page) Cell Host & Microbe 33, 408–419.e1–e8, March 12, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: GOT1 cells Dr. Lennart Svensson N/A Human: HT-29 cells ATCC Cat# HTB38 Experimental models: Organisms/strains 129S1/SvImJ The Jackson Laboratory Strain #: 002448 Ifnar1-/- Ifngr1-/- Virgin laboratory39 N/A 129S6/SvEv-Stat1tm1Rds Taconic Biosciences Strain #: 2045 C57BL/6J The Jackson Laboratory Strain #: 000664 B6.Cg-Ifngr1tm1Agt Ifnar1tm1.2Ees/J The Jackson Laboratory Strain #: 029098 Ifnar1-/- Ifnlr1-/- Kotenko laboratory40 N/A B6.129S7-Ifngr1tm1Agt/J The Jackson Laboratory Strain #: 003288 Ifnlr1-/- Kotenko laboratory40 N/A Ifnlr1fl/fl Ding laboratory N/A Ifnlr1fl/fl Vil-Cre Ding laboratory N/A Mavsfl/fl Baldridge laboratory N/A Mavsfl/fl Alpi-CreERT2 Baldridge laboratory N/A Oligonucleotides Home designed qRT-PCR primers, see Table S1 This paper N/A Mouse Nlrc5 TaqMan primer Thermo Fisher Scientific Cat# Mm01243039_m1 Mouse Tnfsf10 TaqMan primer Thermo Fisher Scientific Cat# Mm01283606_m1 Software and algorithms PRISM (version 10.3.0) Graphpad https://www.graphpad.com FIJI (version 2.14.0/1.54f) ImageJ https://fiji.sc Other Transwells Corning Cat# 3413

    Reverse Transcription:

    Article Title: Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-streptomycin-L-glutamine (100X) Gibco Cat# 10378016 Amphotericin B Sigma-Aldrich Cat# A9528 RPMI 1640 medium Gibco Cat# 11875093 Insulin solution human Sigma-Aldrich Cat# I9278 Apo-transferrin human Sigma-Aldrich Cat# T2036 Advanced DMEM F12 Gibco Cat# 12634010 HEPES Gibco Cat# 15630106 Non-essential amino acids solution Gibco Cat# 11140050 Sodium pyruvate Gibco Cat# 11360070 Trypsin Sigma-Aldrich Cat# T4799 Sucrose Sigma-Aldrich Cat# S0389 DPBS, calcium, magnesium Gibco Cat# 14040133 Cesium chloride Sigma-Aldrich Cat# 20996 SeaKem ME agarose Lonza Cat# 50010 Medium 199 (10X) Gibco Cat# 11825015 Sodium bicarbonate Gibco Cat# 25080094 Neutral red solution Sigma-Aldrich Cat# N2889 BACTO agar BD Cat# 214010 Psoralen Sigma-Aldrich Cat# P8399 Ondansetron Selleckchem Cat# S1390 Formalin 10% Neutral Buffered Solution Research Products International Cat# F10800 Tissue-Tek O.C.T. .. Compound SAKURA Cat# 4583 Albumin, Bovine Fraction V [BSA] Research Products International Cat# A30075 Triton X-100 Sigma-Aldrich Cat# T8787 DL-dithiothreitol Sigma-Aldrich Cat# 43815 UltraPure 0.5M EDTA Invitrogen Cat# 15575020 Matrigel Corning Cat# 356234 Y-27632 dihydrochloride R&D Cat# 1254 SB 431542 R&D Cat# 1614 Gastrin Sigma-Aldrich Cat# G9145 A83-01 Sigma-Aldrich Cat# SML0788 DAPT Selleckchem Cat# S2215 Mouse EGF R&D Cat# 2028-EG Paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 15710 Collagen Sigma-Aldrich Cat# C5533 TrypLE Express Enzyme Gibco Cat# 12604013 Human EGF Invitrogen Cat# A42556 Critical commercial assays The human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA kit R&D Systems Cat# DY1598B05 Serotonin ELISA kit IBL International Cat# RE59121 RNeasy Plus Mini Kit QIAGEN Cat# 74136 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 SYBR Green qPCR ReadyMix Sigma-Aldrich Cat# KCQS00 TaqMan assays Applied Biosystems Cat# 4444557 Deposited data scRNA-seq datasets This study GSE288639 Experimental models: Cell lines African green monkey: MA104 cells ATCC Cat# CRL-2378.1 Mouse: L929 cells ATCC Cat# CCL-1 (Continued on next page) Cell Host & Microbe 33, 408–419.e1–e8, March 12, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: GOT1 cells Dr. Lennart Svensson N/A Human: HT-29 cells ATCC Cat# HTB38 Experimental models: Organisms/strains 129S1/SvImJ The Jackson Laboratory Strain #: 002448 Ifnar1-/- Ifngr1-/- Virgin laboratory39 N/A 129S6/SvEv-Stat1tm1Rds Taconic Biosciences Strain #: 2045 C57BL/6J The Jackson Laboratory Strain #: 000664 B6.Cg-Ifngr1tm1Agt Ifnar1tm1.2Ees/J The Jackson Laboratory Strain #: 029098 Ifnar1-/- Ifnlr1-/- Kotenko laboratory40 N/A B6.129S7-Ifngr1tm1Agt/J The Jackson Laboratory Strain #: 003288 Ifnlr1-/- Kotenko laboratory40 N/A Ifnlr1fl/fl Ding laboratory N/A Ifnlr1fl/fl Vil-Cre Ding laboratory N/A Mavsfl/fl Baldridge laboratory N/A Mavsfl/fl Alpi-CreERT2 Baldridge laboratory N/A Oligonucleotides Home designed qRT-PCR primers, see Table S1 This paper N/A Mouse Nlrc5 TaqMan primer Thermo Fisher Scientific Cat# Mm01243039_m1 Mouse Tnfsf10 TaqMan primer Thermo Fisher Scientific Cat# Mm01283606_m1 Software and algorithms PRISM (version 10.3.0) Graphpad https://www.graphpad.com FIJI (version 2.14.0/1.54f) ImageJ https://fiji.sc Other Transwells Corning Cat# 3413

    SYBR Green Assay:

    Article Title: Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-streptomycin-L-glutamine (100X) Gibco Cat# 10378016 Amphotericin B Sigma-Aldrich Cat# A9528 RPMI 1640 medium Gibco Cat# 11875093 Insulin solution human Sigma-Aldrich Cat# I9278 Apo-transferrin human Sigma-Aldrich Cat# T2036 Advanced DMEM F12 Gibco Cat# 12634010 HEPES Gibco Cat# 15630106 Non-essential amino acids solution Gibco Cat# 11140050 Sodium pyruvate Gibco Cat# 11360070 Trypsin Sigma-Aldrich Cat# T4799 Sucrose Sigma-Aldrich Cat# S0389 DPBS, calcium, magnesium Gibco Cat# 14040133 Cesium chloride Sigma-Aldrich Cat# 20996 SeaKem ME agarose Lonza Cat# 50010 Medium 199 (10X) Gibco Cat# 11825015 Sodium bicarbonate Gibco Cat# 25080094 Neutral red solution Sigma-Aldrich Cat# N2889 BACTO agar BD Cat# 214010 Psoralen Sigma-Aldrich Cat# P8399 Ondansetron Selleckchem Cat# S1390 Formalin 10% Neutral Buffered Solution Research Products International Cat# F10800 Tissue-Tek O.C.T. .. Compound SAKURA Cat# 4583 Albumin, Bovine Fraction V [BSA] Research Products International Cat# A30075 Triton X-100 Sigma-Aldrich Cat# T8787 DL-dithiothreitol Sigma-Aldrich Cat# 43815 UltraPure 0.5M EDTA Invitrogen Cat# 15575020 Matrigel Corning Cat# 356234 Y-27632 dihydrochloride R&D Cat# 1254 SB 431542 R&D Cat# 1614 Gastrin Sigma-Aldrich Cat# G9145 A83-01 Sigma-Aldrich Cat# SML0788 DAPT Selleckchem Cat# S2215 Mouse EGF R&D Cat# 2028-EG Paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 15710 Collagen Sigma-Aldrich Cat# C5533 TrypLE Express Enzyme Gibco Cat# 12604013 Human EGF Invitrogen Cat# A42556 Critical commercial assays The human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA kit R&D Systems Cat# DY1598B05 Serotonin ELISA kit IBL International Cat# RE59121 RNeasy Plus Mini Kit QIAGEN Cat# 74136 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 SYBR Green qPCR ReadyMix Sigma-Aldrich Cat# KCQS00 TaqMan assays Applied Biosystems Cat# 4444557 Deposited data scRNA-seq datasets This study GSE288639 Experimental models: Cell lines African green monkey: MA104 cells ATCC Cat# CRL-2378.1 Mouse: L929 cells ATCC Cat# CCL-1 (Continued on next page) Cell Host & Microbe 33, 408–419.e1–e8, March 12, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: GOT1 cells Dr. Lennart Svensson N/A Human: HT-29 cells ATCC Cat# HTB38 Experimental models: Organisms/strains 129S1/SvImJ The Jackson Laboratory Strain #: 002448 Ifnar1-/- Ifngr1-/- Virgin laboratory39 N/A 129S6/SvEv-Stat1tm1Rds Taconic Biosciences Strain #: 2045 C57BL/6J The Jackson Laboratory Strain #: 000664 B6.Cg-Ifngr1tm1Agt Ifnar1tm1.2Ees/J The Jackson Laboratory Strain #: 029098 Ifnar1-/- Ifnlr1-/- Kotenko laboratory40 N/A B6.129S7-Ifngr1tm1Agt/J The Jackson Laboratory Strain #: 003288 Ifnlr1-/- Kotenko laboratory40 N/A Ifnlr1fl/fl Ding laboratory N/A Ifnlr1fl/fl Vil-Cre Ding laboratory N/A Mavsfl/fl Baldridge laboratory N/A Mavsfl/fl Alpi-CreERT2 Baldridge laboratory N/A Oligonucleotides Home designed qRT-PCR primers, see Table S1 This paper N/A Mouse Nlrc5 TaqMan primer Thermo Fisher Scientific Cat# Mm01243039_m1 Mouse Tnfsf10 TaqMan primer Thermo Fisher Scientific Cat# Mm01283606_m1 Software and algorithms PRISM (version 10.3.0) Graphpad https://www.graphpad.com FIJI (version 2.14.0/1.54f) ImageJ https://fiji.sc Other Transwells Corning Cat# 3413

    Real-time Polymerase Chain Reaction:

    Article Title: Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-streptomycin-L-glutamine (100X) Gibco Cat# 10378016 Amphotericin B Sigma-Aldrich Cat# A9528 RPMI 1640 medium Gibco Cat# 11875093 Insulin solution human Sigma-Aldrich Cat# I9278 Apo-transferrin human Sigma-Aldrich Cat# T2036 Advanced DMEM F12 Gibco Cat# 12634010 HEPES Gibco Cat# 15630106 Non-essential amino acids solution Gibco Cat# 11140050 Sodium pyruvate Gibco Cat# 11360070 Trypsin Sigma-Aldrich Cat# T4799 Sucrose Sigma-Aldrich Cat# S0389 DPBS, calcium, magnesium Gibco Cat# 14040133 Cesium chloride Sigma-Aldrich Cat# 20996 SeaKem ME agarose Lonza Cat# 50010 Medium 199 (10X) Gibco Cat# 11825015 Sodium bicarbonate Gibco Cat# 25080094 Neutral red solution Sigma-Aldrich Cat# N2889 BACTO agar BD Cat# 214010 Psoralen Sigma-Aldrich Cat# P8399 Ondansetron Selleckchem Cat# S1390 Formalin 10% Neutral Buffered Solution Research Products International Cat# F10800 Tissue-Tek O.C.T. .. Compound SAKURA Cat# 4583 Albumin, Bovine Fraction V [BSA] Research Products International Cat# A30075 Triton X-100 Sigma-Aldrich Cat# T8787 DL-dithiothreitol Sigma-Aldrich Cat# 43815 UltraPure 0.5M EDTA Invitrogen Cat# 15575020 Matrigel Corning Cat# 356234 Y-27632 dihydrochloride R&D Cat# 1254 SB 431542 R&D Cat# 1614 Gastrin Sigma-Aldrich Cat# G9145 A83-01 Sigma-Aldrich Cat# SML0788 DAPT Selleckchem Cat# S2215 Mouse EGF R&D Cat# 2028-EG Paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 15710 Collagen Sigma-Aldrich Cat# C5533 TrypLE Express Enzyme Gibco Cat# 12604013 Human EGF Invitrogen Cat# A42556 Critical commercial assays The human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA kit R&D Systems Cat# DY1598B05 Serotonin ELISA kit IBL International Cat# RE59121 RNeasy Plus Mini Kit QIAGEN Cat# 74136 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 SYBR Green qPCR ReadyMix Sigma-Aldrich Cat# KCQS00 TaqMan assays Applied Biosystems Cat# 4444557 Deposited data scRNA-seq datasets This study GSE288639 Experimental models: Cell lines African green monkey: MA104 cells ATCC Cat# CRL-2378.1 Mouse: L929 cells ATCC Cat# CCL-1 (Continued on next page) Cell Host & Microbe 33, 408–419.e1–e8, March 12, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: GOT1 cells Dr. Lennart Svensson N/A Human: HT-29 cells ATCC Cat# HTB38 Experimental models: Organisms/strains 129S1/SvImJ The Jackson Laboratory Strain #: 002448 Ifnar1-/- Ifngr1-/- Virgin laboratory39 N/A 129S6/SvEv-Stat1tm1Rds Taconic Biosciences Strain #: 2045 C57BL/6J The Jackson Laboratory Strain #: 000664 B6.Cg-Ifngr1tm1Agt Ifnar1tm1.2Ees/J The Jackson Laboratory Strain #: 029098 Ifnar1-/- Ifnlr1-/- Kotenko laboratory40 N/A B6.129S7-Ifngr1tm1Agt/J The Jackson Laboratory Strain #: 003288 Ifnlr1-/- Kotenko laboratory40 N/A Ifnlr1fl/fl Ding laboratory N/A Ifnlr1fl/fl Vil-Cre Ding laboratory N/A Mavsfl/fl Baldridge laboratory N/A Mavsfl/fl Alpi-CreERT2 Baldridge laboratory N/A Oligonucleotides Home designed qRT-PCR primers, see Table S1 This paper N/A Mouse Nlrc5 TaqMan primer Thermo Fisher Scientific Cat# Mm01243039_m1 Mouse Tnfsf10 TaqMan primer Thermo Fisher Scientific Cat# Mm01283606_m1 Software and algorithms PRISM (version 10.3.0) Graphpad https://www.graphpad.com FIJI (version 2.14.0/1.54f) ImageJ https://fiji.sc Other Transwells Corning Cat# 3413

    other:

    Article Title: Itaconate promotes inflammatory responses in tissue-resident alveolar macrophages and exacerbates acute lung injury.
    Article Snippet: Article Itaconate promotes inflammatory responses in tissue-resident alveolar macrophages and exacerbates acute lung injury Graphical abstract Highlights • Itaconate enhances cytokine production and NLRP3 activation in alveolar macrophages • Dimethyl and 4-octyl itaconate oppose native itaconate’s effects on alveolar macrophages • The alveolar microenvironment controls AM responsiveness to itaconate • Itaconate worsens lipopolysaccharide-induced acute lung injury Authors Mengtian Shan, Song Zhang, Zhibing Luo, ..., Qiang Li, Zhongliang Guo, Feilong Wang Correspondence liqressh1962@163.com (Q.L.), drguozhl@163.com (Z.G.), wang_feilong@tongji.edu.cn (F.W.). In brief Shan et al. demonstrate that itaconate promotes proinflammatory cytokine production and NLRP3 inflammasome activation in alveolar macrophages, contrasting its anti-inflammatory role in BMDMs.. Itaconate treatment exacerbates lung injury in vivo, highlighting the need to explore its immunomodulatory effects across tissue-resident macrophages before clinical application.



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